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Biochemistry Insights

Fast and Simple micro-RNA Northern Blots

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Biochemistry Insights 2009:2 1-3

Methodology

Published on 04 Mar 2009


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Abstract

RNA northern blots provide robust measurements of gene expression. The simple northern blot technique described in this report has been optimised to provide rapid, reproducible detection and analysis of mature and precursor forms of microRNAs. This protocol economises on the use of commercially available components and secondly reduces the hybridisation step to 2 hours.



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