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Biochemistry Insights

Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli

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Biochemistry Insights 2010:3 41-46

Methodology

Published on 24 Aug 2010

DOI: 10.4137/BCI.S5123


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Abstract

A synthetic gene encoding bovine terminal deoxynucleotidyl transferase (TdT) was generated, cloned into an expression vector and expressed in E.coli. The effects of altering culture and induction conditions on the nature of recombinant protein production were investigated. This led to the expression of active recombinant bovine TdT in E.coli. After purification and characterisation, the activity of the enzyme was assessed in a biological assay for apoptosis. The process described in this report enables the economical production of TdT for high throughput applications.



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