Evolutionary Bioinformatics

Intraspecific ITS Variability in the Kingdom Fungi as Expressed in the International Sequence Databases and Its Implications for Molecular Species Identification

Submit a Paper

Evolutionary Bioinformatics 2008:4 193-201

Published on 26 May 2008

DOI: 10.4137/EBO.S653

Further metadata provided in PDF

Sign up for email alerts to receive notifications of new articles published in Evolutionary Bioinformatics

R. Henrik Nilsson1, Erik Kristiansson2, Martin Ryberg1, Nils Hallenberg1 and Karl-Henrik Larsson1

1Department of Plant and Environmental Sciences, University of Gothenburg, Box  461, 405 30 Göteborg, Sweden. 2Mathematical Statistics, Chalmers University of Technology/Department of Zoology, University of Gothenburg, 412 96 Göteborg, Sweden.


The internal transcribed spacer (ITS) region of the nuclear ribosomal repeat unit is the most popular locus for species identification and subgeneric phylogenetic inference in sequence-based mycological research. The region is known to show certain variability even within species, although its intraspecific variability is often held to be limited and clearly separated from interspecific variability. The existence of such a divide between intra- and interspecific variability is implicitly assumed by automated approaches to species identification, but whether intraspecific variability indeed is negligible within the fungal kingdom remains contentious. The present study estimates the intraspecific ITS variability in all fungi presently available to the mycological community through the international sequence databases. Substantial differences were found within the kingdom, and the results are not easily correlated to the taxonomic affiliation or nutritional mode of the taxa considered. No single unifying yet stringent upper limit for intraspecific variability, such as the canonical 3% threshold, appears to be applicable with the desired outcome throughout the fungi. Our results caution against simplified approaches to automated ITS-based species delimitation and reiterate the need for taxonomic expertise in the translation of sequence data into species names.




BibTex citation   (BIBDESK, LATEX)


What Your Colleagues Say About Evolutionary Bioinformatics
It was a nice experience for me to publish my first paper in Evolutionary Bioinformatics.  The peer review process was fast, critical, helpful and fair. The production process was also fast and accurate. Thanks for your hard work.
Dr Kangquan Yin (Peking University, Beijing, PRC)
More Testimonials

Quick Links

New article and journal news notification services